Original Papers

 

Coordination of cohesin and DNA replication observed with purified proteins

Yasuto Murayama, Shizuko Endo, Yumiko Kurokawa, Ayako Kurita, Sanae Iwasaki, Hiroyuki Araki

Nature  626(7999) 653-660 (2024)

 

Homology length dictates the requirement for Rad51 and Rad52 in gene targeting in the Basidiomycota yeast Naganishia liquefaciens

Maierdan Palihati, Hideo Tsubouchi, Bilge Argunhan, Rei Kajitani, Omirgul Bakenova, Yong-Woon Han, Yasuto Murayama, Takehiko Itoh, Hiroshi Iwasaki

Current Genetics  67(6) 919-936 (2021)

 

Rrp1 translocase and ubiquitin ligase activities restrict the genome destabilising effects of Rad51 in fission yeast

Jakub Muraszko, Karol Kramarz, Bilge Argunhan, Kentaro Ito, Gabriela Baranowska, Yumiko Kurokawa, Yasuto Murayama, Hideo Tsubouchi, Sarah Lambert, Hiroshi Iwasaki, Dorota Dziadkowiec

Nucleic Acids Research  49(12) 6832-6848 (2021)

 

A conserved Ctp1/CtIP C-terminal peptide stimulates Mre11 endonuclease activity

Aleksandar Zdravković, James M Daley, Arijit Dutta, Tatsuya Niwa, Yasuto Murayama, Shuji Kanamaru, Kentaro Ito, Takahisa Maki, Bilge Argunhan, Masayuki Takahashi, Hideo Tsubouchi, Patrick Sung, Hiroshi Iwasaki

Proceedings of the National Academy of Sciences of the United States of America   118(11) (2021)

 

DNA binding by the Mis4^Scc2 loader promotes topological DNA entrapment by the cohesin ring

Yumiko Kurokawa, Yasuto Murayama

Cell Reports  33(6) 108357 (2020)

 

Real-time tracking reveals catalytic roles for the two DNA binding sites of Rad51

Kentaro Ito, Yasuto Murayama, Yumiko Kurokawa, Shuji Kanamaru, Yuichi Kokabu, Takahisa Maki, Tsutomu Mikawa, Bilge Argunhan, Hideo Tsubouchi, Mitsunori Ikeguchi, Masayuki Takahashi, Hiroshi Iwasaki

Nature Communications  11(1) 2950-2950 (2020)

 

Cooperative interactions facilitate stimulation of Rad51 by the Swi5-Sfr1 auxiliary factor complex 

Bilge Argunhan, Masayoshi Sakakura, Negar Afshar, Misato Kurihara, Kentaro Ito, Takahisa Maki, Shuji Kanamaru, Yasuto Murayama, Hideo Tsubouchi, Masayuki Takahashi, Hideo Takahashi, Hiroshi Iwasaki

eLife  9 e52566 (2020)

 

Establishment of DNA-DNA interactions by the cohesin ring

Yasuto Murayama, Catarina P Samora, Yumiko Kurokawa, Hiroshi Iwasaki, Frank Uhlmann

Cell  172(3) 465-477 (2018)

 

Two three-strand intermediates are processed during Rad51-driven DNA strand exchange 

Kentaro Ito, Yasuto Murayama, Masayuki Takahashi, Hiroshi Iwasaki

Nature Structural and Molecular Biology  25(1) 29-36 (2018)

 

Fundamental cell cycle kinases collaborate to ensure timely destruction of the synaptonemal complex during meiosis 

Bilge Argunhan, Wing-Kit Leung, Negar Afshar, Yaroslav Terentyev, Vijayalakshmi V. Subramanian, Yasuto Murayama, Andreas Hochwagen, Hiroshi Iwasaki, Tomomi Tsubouchi, Hideo Tsubouchi

EMBO journal  36(17) 2488-2509 (2017)

 

In vitro site-specific recombination mediated by the tyrosine recombinase XerA of Thermoplasma acidophilum 

Minji Jo, Yasuto Murayama, Yasuhiro Tsutsui, Hiroshi Iwasaki

Genes to Cells  22(7) 646-661 (2017)

 

Structure of the cohesin loader Scc2 

William C. H. Chao, Yasuto Murayama, Sofia Munoz, Andrew W. Jones, Benjamin O. Wade, Andrew G. Purkiss, Xiao-Wen Hu, Aaron Borg, Ambrosius P. Snijders, Frank Uhlmann, Martin R. Singleton

Nature communications  8 13952 (2017)

 

DNA entry into and exit out of the cohesin ring by an interlocking gate mechanism 

Yasuto Murayama, Frank Uhlmann

Cell  163(7) 1628-1640 (2015)

 

Structural studies reveal the functional modularity of the Scc2-Scc4 cohesin loader 

William C. H. Chao, Yasuto Murayama, Sofia Munoz, Alessandro Costa, Frank Uhlmann, Martin R. Singleton

Cell reports  12(5) 719-725 (2015)

 

Biochemical reconstitution of topological DNA binding by the cohesin ring 

Yasuto Murayama, Frank Uhlmann

Nature  505(7483) 367-371 (2014)

 

Dual regulation of Dmc1-driven DNA strand exchange by Swi5-Sfr1 activation and Rad22 inhibition 

Yasuto Murayama, Yumiko Kurokawa, Yasuhiro Tsutsui, Hiroshi Iwasaki

Genes & Development  27(21) 2299-2304 (2013)

 

Mechanistic insights into the activation of Rad51-mediated strand exchange from the structure of a recombination activator, the Swi5-Sfr1 complex 

Naoyuki Kuwabara, Yasuto Murayama, Hiroshi Hashimoto, Yuuichi Kokabu, Mitsunori Ikeguchi, Mamoru Sato, Kouta Mayanagi, Yasuhiro Tsutsui, Hiroshi Iwasaki, Toshiyuki Shimizu

Structure  20(3) 440-449 (2012)

 

Fission yeast Swi5-Sfr1 protein complex, an activator of Rad51 recombinase, forms an extremely elongated dogleg-shaped structure 

Yuichi Kokabu, Yasuto Murayama, Naoyuki Kuwabara, Tomotaka Oroguchi, Hiroshi Hashimoto, Yasuhiro Tsutsui, Naohito Nozaki, Satoko Akashi, Satoru Unzai, Toshiyuki Shimizu, Hiroshi Iwasaki, Mamoru Sato, Mitsunori Ikeguchi

Journal of Biological Chemistry  286(50) 43569-43576 (2011)

 

The fission yeast meiosis-specific Dmc1 recombinase mediates formation and branch migration of Holliday junctions by preferentially promoting strand exchange in a direction opposite to that of Rad51 

Yasuto Murayama, Yasuhiro Tsutsui, Hiroshi Iwasaki

Genes & Development  25(5) 516-527 (2011)

 

Molecular characterization of the role of the Schizosaccharomyces pombe nip1(+)/ctp1(+) gene in DNA double-strand break repair in association with the Mre11-Rad50-Nbs1 complex 

Yufuko Akamatsu, Yasuto Murayama, Takatomi Yamada, Tomofumi Nakazaki, Yasuhiro Tsutsui, Kunihiro Ohta, Hiroshi Iwasaki

Molecular and Cellular Biology  28(11) 3639-3651 (2008)  

 

Reconstitution of DNA strand exchange mediated by Rhp51 recombinase and two mediators 

Yumiko Kurokawa, Yasuto Murayama, Nami Haruta-Takahashi, Itaru Urabe, Hiroshi Iwasaki

PLOS Biology  6(4) 836-848 (2008)    

 

Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases 

Yasuto Murayama, Yumiko Kurokawa, Kouta Mayanagi, Hiroshi Iwasaki

Nature  451(7181) 1018-1021 (2008)  

 

The Swi5-Sfr1 complex stimulates Rhp51/Rad51- and Dmc1-mediated DNA strand exchange in vitro

Nami Haruta, Yumiko Kurokawa, Yasuto Murayama, Yufuko Akamatsu, Satoru Unzai, Yasuhiro Tsutsui, Hiroshi Iwasaki

Nature structural & molecular biology  13(9) 823-830(2006)   

Review articles

 

DNA entry, exit and second DNA capture by cohesin: insights from biochemical experiments

Murayama Y

Nucleus (Austin, Tex.)  9(1) 492-502 (2018)

 

The differentiated and conserved roles of Swi5-Sfr1 in homologous recombination 

Bilge Argunhan, Yasuto Murayama, Hiroshi Iwasaki

FEBS Letters  591(14) 2035-2047 (2017)

 

An in vitro assay for monitoring topological DNA entrapment by the chromosomal cohesin complex 

Yasuto Murayama, Frank Uhlmann

Methods in Molecular Biology 1515 23-35 (2017)

 

Chromosome segregation: how to open cohesin without cutting the ring? 

Murayama Y, Uhlmann F

EMBO journal  32(5) 614-616 (2013)  

 

An in vitro assay for monitoring the formation and branch migration of holliday junctions mediated by a eukaryotic recombinase

Murayama Y, Iwasaki H

Methods in Molecular Biology (Clifton, N.J.)  745 385-405 (2011)

 

Expression, purification and crystallization of Swi5 and the Swi5-Sfr1 complex from fission yeast 

Naoyuki Kuwabara, Hiroshi Hashimoto, Noriyo Yamada, Satoru Unzai, Mitsunori Ikeguchi, Mamoru Sato, Yasuto Murayama, Hiroshi Iwasaki, Toshiyuki Shimizu

Acta Crystallographica Section F, Structural Biology and Crystallization Communications 66(Pt 9) 1124-1126 (2010)  

 

Fission yeast Swi5 protein, a novel DNA recombination mediator 

Nami Haruta, Yufuko Akamatsu, Yasuhiro Tsutsui, Yumiko Kurokawa, Yasuto Murayama, Benoit Arcangioli, Hiroshi Iwasaki

DNA Repair  7(1) 1-9 (2008)