論文等

原著論文

Coordination of cohesin and DNA replication observed with purified proteins

Yasuto Murayama, Shizuko Endo, Yumiko Kurokawa, Ayako Kurita, Sanae Iwasaki, Hiroyuki Araki

Nature 626(7999) 653-660 (2024)

Homology length dictates the requirement for Rad51 and Rad52 in gene targeting in the Basidiomycota yeast Naganishia liquefaciens

Maierdan Palihati, Hideo Tsubouchi, Bilge Argunhan, Rei Kajitani, Omirgul Bakenova, Yong-Woon Han, Yasuto Murayama, Takehiko Itoh, Hiroshi Iwasaki

Current Genetics 67(6) 919-936 (2021)

Rrp1 translocase and ubiquitin ligase activities restrict the genome destabilising effects of Rad51 in fission yeast

Jakub Muraszko, Karol Kramarz, Bilge Argunhan, Kentaro Ito, Gabriela Baranowska, Yumiko Kurokawa, Yasuto Murayama, Hideo Tsubouchi, Sarah Lambert, Hiroshi Iwasaki, Dorota Dziadkowiec

Nucleic Acids Research 49(12) 6832-6848 (2021)

A conserved Ctp1/CtIP C-terminal peptide stimulates Mre11 endonuclease activity

Aleksandar Zdravković, James M Daley, Arijit Dutta, Tatsuya Niwa, Yasuto Murayama, Shuji Kanamaru, Kentaro Ito, Takahisa Maki, Bilge Argunhan, Masayuki Takahashi, Hideo Tsubouchi, Patrick Sung, Hiroshi Iwasaki

Proceedings of the National Academy of Sciences of the United States of America 118(11) (2021)

DNA binding by the Mis4^Scc2 loader promotes topological DNA entrapment by the cohesin ring

Yumiko Kurokawa, Yasuto Murayama

Cell Reports 33(6) 108357 (2020)

Real-time tracking reveals catalytic roles for the two DNA binding sites of Rad51

Kentaro Ito, Yasuto Murayama, Yumiko Kurokawa, Shuji Kanamaru, Yuichi Kokabu, Takahisa Maki, Tsutomu Mikawa, Bilge Argunhan, Hideo Tsubouchi, Mitsunori Ikeguchi, Masayuki Takahashi, Hiroshi Iwasaki

Nature Communications 11(1) 2950 (2020)

Cooperative interactions facilitate stimulation of Rad51 by the Swi5-Sfr1 auxiliary factor complex

Bilge Argunhan, Masayoshi Sakakura, Negar Afshar, Misato Kurihara, Kentaro Ito, Takahisa Maki, Shuji Kanamaru, Yasuto Murayama, Hideo Tsubouchi, Masayuki Takahashi, Hideo Takahashi, Hiroshi Iwasaki

eLife 9 e52566 (2020)

Establishment of DNA-DNA interactions by the cohesin ring

Yasuto Murayama, Catarina P Samora, Yumiko Kurokawa, Hiroshi Iwasaki, Frank Uhlmann

Cell 172(3) 465-477 (2018)

Two three-strand intermediates are processed during Rad51-driven DNA strand exchange

Kentaro Ito, Yasuto Murayama, Masayuki Takahashi, Hiroshi Iwasaki

Nature Structural and Molecular Biology 25(1) 29-36 (2018)

Fundamental cell cycle kinases collaborate to ensure timely destruction of the synaptonemal complex during meiosis

Bilge Argunhan, Wing-Kit Leung, Negar Afshar, Yaroslav Terentyev, Vijayalakshmi V. Subramanian, Yasuto Murayama, Andreas Hochwagen, Hiroshi Iwasaki, Tomomi Tsubouchi, Hideo Tsubouchi

EMBO journal 36(17) 2488-2509 (2017)

In vitro site-specific recombination mediated by the tyrosine recombinase XerA of Thermoplasma acidophilum

Minji Jo, Yasuto Murayama, Yasuhiro Tsutsui, Hiroshi Iwasaki

Genes to Cells 22(7) 646-661 (2017)

Structure of the cohesin loader Scc2

William C. H. Chao, Yasuto Murayama, Sofia Munoz, Andrew W. Jones, Benjamin O. Wade, Andrew G. Purkiss, Xiao-Wen Hu, Aaron Borg, Ambrosius P. Snijders, Frank Uhlmann, Martin R. Singleton

Nature communications 8 13952 (2017)

DNA entry into and exit out of the cohesin ring by an interlocking gate mechanism

Yasuto Murayama, Frank Uhlmann

Cell 163(7) 1628-1640 (2015)

Structural studies reveal the functional modularity of the Scc2-Scc4 cohesin loader

William C. H. Chao, Yasuto Murayama, Sofia Munoz, Alessandro Costa, Frank Uhlmann, Martin R. Singleton

Cell reports 12(5) 719-725 (2015)

Biochemical reconstitution of topological DNA binding by the cohesin ring

Yasuto Murayama, Frank Uhlmann

Nature 505(7483) 367-371 (2014)

Dual regulation of Dmc1-driven DNA strand exchange by Swi5-Sfr1 activation and Rad22 inhibition

Yasuto Murayama, Yumiko Kurokawa, Yasuhiro Tsutsui, Hiroshi Iwasaki

Genes & Development 27(21) 2299-2304 (2013)

Mechanistic Insights into the activation of Rad51-mediated strand exchange from the structure of a recombination activator, the Swi5-Sfr1 complex

Naoyuki Kuwabara, Yasuto Murayama, Hiroshi Hashimoto, Yuuichi Kokabu, Mitsunori Ikeguchi, Mamoru Sato, Kouta Mayanagi, Yasuhiro Tsutsui, Hiroshi Iwasaki, Toshiyuki Shimizu

Structure 20(3) 440-449 (2012)

Fission yeast Swi5-Sfr1 protein complex, an activator of Rad51 recombinase, forms an extremely elongated dogleg-shaped structure

Yuichi Kokabu, Yasuto Murayama, Naoyuki Kuwabara, Tomotaka Oroguchi, Hiroshi Hashimoto, Yasuhiro Tsutsui, Naohito Nozaki, Satoko Akashi, Satoru Unzai, Toshiyuki Shimizu, Hiroshi Iwasaki, Mamoru Sato, Mitsunori Ikeguchi

Journal of Biological Chemistry 286(50) 43569-43576 (2011)

The fission yeast meiosis-specific Dmc1 recombinase mediates formation and branch migration of Holliday junctions by preferentially promoting strand exchange in a direction opposite to that of Rad51

Yasuto Murayama, Yasuhiro Tsutsui, Hiroshi Iwasaki

Genes & Development 25(5) 516-527 (2011)

Molecular characterization of the role of the Schizosaccharomyces pombe nip1(+)/ctp1(+) gene in DNA double-strand break repair in association with the Mre11-Rad50-Nbs1 complex

Yufuko Akamatsu, Yasuto Murayama, Takatomi Yamada, Tomofumi Nakazaki, Yasuhiro Tsutsui, Kunihiro Ohta, Hiroshi Iwasaki

Molecular and Cellular Biology 28(11) 3639-3651 (2008)

Reconstitution of DNA strand exchange mediated by Rhp51 recombinase and two mediators

Yumiko Kurokawa, Yasuto Murayama, Nami Haruta-Takahashi, Itaru Urabe, Hiroshi Iwasaki

PLOS Biology 6(4) 836-848 (2008)

Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases

Yasuto Murayama, Yumiko Kurokawa, Kouta Mayanagi, Hiroshi Iwasaki

Nature 451(7181) 1018-1021 (2008)

The Swi5-Sfr1 complex stimulates Rhp51/Rad51- and Dmc1-mediated DNA strand exchange in vitro

Nami Haruta, Yumiko Kurokawa, Yasuto Murayama, Yufuko Akamatsu, Satoru Unzai, Yasuhiro Tsutsui, Hiroshi Iwasaki

Nature structural & molecular biology 13(9) 823-830(2006)

総説等

DNA entry, exit and second DNA capture by cohesin: insights from biochemical experiments

Murayama Y

Nucleus (Austin, Tex.) 9(1) 492-502 (2018)

The differentiated and conserved roles of Swi5-Sfr1 in homologous recombination

Bilge Argunhan, Yasuto Murayama, Hiroshi Iwasaki

FEBS Letters 591(14) 2035-2047 (2017)

An in vitro assay for monitoring topological DNA entrapment by the chromosomal cohesin complex

Yasuto Murayama, Frank Uhlmann

Methods in Molecular Biology 1515 23-35 (2017)

Chromosome segregation: how to open cohesin without cutting the ring?

Murayama Y, Uhlmann F

EMBO journal 32(5) 614-616 (2013)

An in vitro assay for monitoring the formation and branch migration of holliday junctions mediated by a eukaryotic recombinase

Murayama Y, Iwasaki H

Methods in Molecular Biology (Clifton, N.J.) 745 385-405 (2011)

Expression, purification and crystallization of Swi5 and the Swi5-Sfr1 complex from fission yeast

Naoyuki Kuwabara, Hiroshi Hashimoto, Noriyo Yamada, Satoru Unzai, Mitsunori Ikeguchi, Mamoru Sato, Yasuto Murayama, Hiroshi Iwasaki, Toshiyuki Shimizu

Acta Crystallographica Section F, Structural Biology and Crystallization Communications 66(Pt 9) 1124-1126 (2010)

Fission yeast Swi5 protein, a novel DNA recombination mediator

Nami Haruta, Yufuko Akamatsu, Yasuhiro Tsutsui, Yumiko Kurokawa, Yasuto Murayama, Benoit Arcangioli, Hiroshi Iwasaki

DNA Repair 7(1) 1-9 (2008)